Fig. 5

iPSCs reduced the autophagy and apoptosis in C2C12 myoblasts under hypoxia. A Immunofluorescence staining showing that after exposure to hypoxia for 24 h, LC3 accumulated in C2C12 myoblast perinuclear area, while in the normoxia and iPSCs co-culture groups, LC3 was diffusely distributed in the cytoplasm, (green, LC3; blue, DAPI). B Results of qPCR analysis showing the mRNA levels of autophagy- and apoptosis-related markers in C2C12 myoblasts after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. C, D Western blot results showing the protein levels of autophagy- and apoptosis-related markers in C2C12 myoblasts after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. Statistics were from three independent replicates of the samples and data were expressed as mean ± SEM; the remaining two groups were compared with the hypoxic group, respectively, and P values are the results of one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significant difference. Scale bar = 100 μm. Co: iPSCs and C2C12 co-culture