Fig. 4

iPSCs had no effect on proliferation of C2C12 myoblasts under hypoxic damage. A Immunofluorescence staining showing EdU⁺ cells in C2C12 myoblasts after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. (red, EdU; blue, Hoechst33342). B The percentage of EdU⁺ cells in C2C12 myoblasts. C The flow chart of C2C12 DNA content. D The percentage of C2C12 myoblasts in each part of the cell cycle in after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. E Results of qPCR analysis showing that the mRNA levels of Ccna2, Ccnb1, and Ccne1 had no effect on C2C12 myoblasts after exposure to normoxia or hypoxia for 24 h with or without iPSCs co-culture. Statistics were from three independent replicates of the samples and data were expressed as mean ± SEM; the remaining two groups were compared with the hypoxic group, respectively, and P values are the results of one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001. ns, no significant difference. Scale bar = 100 μm. Co: iPSCs and C2C12 co-culture. EdU: 5-Ethynyl-20-deoxyuridine