Fig. 1
From: iPSCs ameliorate hypoxia-induced autophagy and atrophy in C2C12 myotubes via the AMPK/ULK1 pathway
Identification of iPSCs. A Growth of iPSCs in serum-free, feeder layer-free cell culture medium under phase contrast microscopy. B Karyotype analysis of iPSCs. C Immunofluorescence staining showing that iPSCs expressed pluripotent stem cell markers Oct4, SSEA4, Sox2, TRA-1-81. D iPSCs grew as spherical embryos during spontaneous differentiation in suspension culture under phase contrast microscopy. E Immunofluorescence staining showing that iPSCs spontaneously differentiated into three germ layers, with FOXA2 in the endoderm, TBX1 in the mesoderm, and Nestin in the ectoderm. Scale bar = 100 μm