Fig. 8

Purity of dimeric RBD during a stress stability at 37 °C measured by GF-HPLC and SDS PAGE. A dRBD chromatograms by GF-HPLC during storage at 37 °C. B XY graph of the percentages of the species calculated from the area under the curve of the peaks corresponding to the dRBD. Lots of the dRBD are shown: Point 1: dBD Lot 3 time 0, Point 2: dRBD Lot 3 time 7 days, Point 3: dRBD Lot 3 time 15 days, Point 4: dRBD Lot 3 time 22 days and Point 5: dRBD Lot 3 time 30 days. Percentage of the dRBD are shown: monomer (blue), dimer (red), aggregates (green) and fragmentation (purple). C Electrophoretic profile by SDS-PAGE of the mRBD at different concentrations of stress (Point 1: MWM, Molecular Weight size Marker, Point 2: dRBD Lot 3 time 0, Point 3: mRBD Lot 3 time 7 days, Point 4: mRBD Lot 3 time 15 days, Point 5: mRBD Lot 3 time 22 days and Point 6: mRBD Lot 3 time 30 days)