Fig. 7
From: NEDD4-1 deficiency impairs satellite cell function during skeletal muscle regeneration
Effect of Nedd4-1 ablation in SC upon repeated injury and regeneration. A Schematic of TMX and BaCl2 treatment to induce consecutive injury in TA muscles. Samples were collected 30 days after the second injury (30 dpi; 52 days) for further analysis. B H&E staining of contra-lateral (uninjured) and injured (30 dpi) TAs from SC-N4wt and SC-N4KO mice. Scale bar = 200 μm. C Quantification shows the average TA weights ± SEM (normalized by total body weight) from SC-N4wt (white) and SC-N4KO (black bars) at 30 dpi. D Quantification shows the average whole TA CSA ± SEM from SC-N4wt (white) and SC-N4KO (black bars) at 30 dpi. For C and D, n = 3, ***P < 0.001. E Quantification shows individual fiber CSA for regenerating (centrally located nuclei) myofibers. Uninjured contralateral myofiber CSA are shown as internal control. mean values are indicated for each condition. n = 3, ***P < 0.001. F TA cryosections from SC-N4wtmT/mG and SC-N4KOmT/mG Cre-reporter mice after 30 days of BaCl2-induced injury (30 dpi). mGFP(+) myofibers show contribution of recombined SCs to muscle regeneration. Representative of 3 independent experiments. Scale bar = 200 μm. G Differences in regenerating diameter from SC-N4mT/mG Cre-reporter mice treated with vehicle (veh; centrally located nuclei non-recombined GFP−) or tamoxifen (TMX; centrally located nuclei/GFP+). Quantification shows individual fiber CSA, and mean values are indicated for each condition. n = 3, ***P < 0.001. Scale bar = 100 μm