Fig. 7
UDCA decreases p70S6K and 4EBPP1 phosphorylation and p70S6K total protein levels into C2C12 myotubes. C2C12 myoblasts differentiated for 4–5 days were incubated with 200 μM UDCA for 72 h. a p70S6K phosphorylation and total levels were detected by western blot analysis, using p70S6K total levels or GAPDH as a loading control, respectively. Molecular weight is indicated in kDa. b Densitometric analysis of p-p70S6K (Thr389) and (c) p70S6K total protein levels were performed. d 4EBP1 phosphorylation and total levels were detected by western blot analysis, using 4EBP1 total levels or β‐actin as a loading control, respectively. Molecular weight is indicated in kDa. e Densitometric analysis of p-4EBP1 (Ser65) and f 4EBP1 total protein levels was performed. The values are shown as a fold of change and expressed as the mean ± SD of three independent experiments (no paired t-test, *p < 0.05 with respect to the control group). SD standard deviation, UDCA ursodeoxycholic acid