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Fig. 6 | Biological Research

Fig. 6

From: Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic flux

Fig. 6

UDCA decreases puromycin incorporation into C2C12 myotubes. C2C12 myoblasts were differentiated for 4–5 days and incubated with 200 μM UDCA for 24 h. a Puromycin incorporation was detected by western blot analysis, using β‐actin as a loading control. Molecular weight is indicated in kDa. b A densitometric analysis of the puromycin incorporation bands was performed. The values are shown as a fold of change and expressed as the mean ± SD of three independent experiments (no paired t-test, *p < 0.05 with respect to the control group). SD standard deviation, UDCA ursodeoxycholic acid

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