Fig. 6From: Ursodeoxycholic acid induces sarcopenia associated with decreased protein synthesis and autophagic fluxUDCA decreases puromycin incorporation into C2C12 myotubes. C2C12 myoblasts were differentiated for 4–5 days and incubated with 200 μM UDCA for 24 h. a Puromycin incorporation was detected by western blot analysis, using β‐actin as a loading control. Molecular weight is indicated in kDa. b A densitometric analysis of the puromycin incorporation bands was performed. The values are shown as a fold of change and expressed as the mean ± SD of three independent experiments (no paired t-test, *p < 0.05 with respect to the control group). SD standard deviation, UDCA ursodeoxycholic acidBack to article page