Fig. 5

Isolated muscle fiber diameter is reduced by UDCA treatment. Muscle fibers were isolated from C57BL/6 male mice and incubated with 200 μM UDCA for 72 h. a Indirect immunofluorescence detected EDL muscle fiber MHC to delimit the fiber diameter. Fluorescent microscopy images were captured. The scale bar indicates 50 μm. b The diameter of the isolated muscle fiber was measured using ImageJ software. The quantification was performed, and the individual values for fiber were plotted. c FDB muscle fiber MHC was detected by indirect immunofluorescence to delimit the fiber diameter. Fluorescent microscopy images were captured. The scale bar indicates 50 μm. d The diameter of the isolated muscle fiber was measured using ImageJ software. The quantification was performed, and the individual values for fiber were plotted. The result shows the value of each fiber, with the mean ± SD for each group (n = 27 EDL muscle fibers, 60 FDB muscle fibers, no paired t-test with Welch’s correction, *p < 0.05 with respect to the control group). EDL extensor digitorum longus, FDB flexor digitorum brevis, MHC myosin heavy chain, SD standard deviation, UDCA ursodeoxycholic acid