Fig. 4
UDCA reduces sarcomeric proteins in C2C12 myotubes. C2C12 myoblasts were differentiated for 4–5 days and incubated with 0, 50, 100, 200, and 400 μM UDCA for 72 h. a MHC and troponin I protein levels were detected by western blot analysis, using β‐actin as the loading control. Molecular weights are shown in kDa. Densitometry analysis for (b) MHC and (c) troponin I is represented as a fold of change expressed as the mean ± SD of three independent experiments (no paired t-test, *p < 0.05 with respect to the control group). MHC myosin heavy chain, SD standard deviation, UDCA ursodeoxycholic acid