Fig. 3
UDCA reduces the diameter of C2C12 myotubes in a dose-dependent manner. Myotubes were incubated with 0–50–100–200 μM UDCA for 72 h. a MHC was detected by indirect immunofluorescence and used to delimit the myotube diameter. Images were captured by fluorescence microscopy. The scale bar indicates 100 μm. b The diameter of the myotubes was measured using ImageJ software. The quantification was performed, and the individual values for myotube were plotted. c The myotubes were classified using the control group median (32.13 μm). Myotubes with large or small diameters were determined if their diameter was larger or smaller than the threshold value. The values indicate the percentage of myotubes expressed as the mean ± SEM of three independent experiments (one‐way ANOVA, post‐hoc Dunnet, *p < 0.05 with respect to the control). ANOVA analysis of variance, MHC myosin heavy chain, SEM standard error of the mean, UDCA ursodeoxycholic acid