Fig. 2

After 6 weeks, UDCA treatment diminishes the fiber diameter and troponin I level in adult mice. C57BL/6 male mice were treated orally with UDCA 200 mg/kg corporal body weight for 6 weeks (1.04% NaCl pH 8.4 to the control group). Mice were monitored daily and were euthanized during the sixth week. a TA muscle cross-sections were stained with laminin to delimit the sarcolemma. The scale bar indicates 100 μm. b The minimal Feret’s diameters were calculated using the MyoVision software. Fiber diameters were grouped from 5 to 80 μm to quantify the total fiber percentage in each group. c Accumulative frequency analysis to UDCA and control group were plotted. d The area under the curve was calculated in accumulative frequency to fiber diameters using GraphPad Prism 8.0 software. e TA muscle was homogenized to evaluate troponin I protein levels by western blot analysis, using β-actin as the loading control, and the molecular weights are depicted in kilodaltons (kDa). f Quantitative analysis for troponin I protein levels is shown. The result shows the mean ± SEM for each group (n = 5 mice per group, no paired t-test, *p < 0.05 with respect to the control group). SEM standard error of the mean, UDCA ursodeoxycholic acid