Fig. 1

Muscle function declines in mice treated with UDCA after 6 weeks. C57BL/6 male mice were treated orally with UDCA 200 mg/kg corporal body weight for 6 weeks (1.04% NaCl pH 8.4 to the control group). Mice were monitored daily, and muscular evaluations were made at the beginning and during the sixth week. a The hindlimb strength was measured by a dynamometer. Values represent the difference between the initial measure before UDCA treatment and the end of treatment. b The forelimb strength was measured by a dynamometer. Values represent the difference between the initial measure before UDCA treatment and the end of treatment. c The forelimb strength was measured by a weightlifting test. Values represent the difference between the initial score normalized before UDCA treatment and the end of treatment. d After euthanizing, the TA muscle was dissected to evaluate the electromyography performance. The area under the curve was calculated for each animal using GraphPad Prism 8.0 software and represented individually. e The maximum distance reached by each animal on a treadmill was recorded. Values represent the difference between the initial distance before UDCA treatment and the end of treatment. The result shows the individual value of the subject, with the mean ± SEM for each group. (n = 5 mice per group, no paired t-test, *p < 0.05 with respect to the control group). SEM standard error of the mean, UDCA ursodeoxycholic acid