Fig. 5

Ambra1 deficiency affects ovary physiology in mice. (A) RT-qPCR analysis of Ambra1 mRNA levels in brain, muscle and ovaries of 3-month-old WT mice. (B, C) RT-qPCR analysis of Ambra1, Stat3 and Myc expression in 3-month-old (B) and 10-month-old (C) Ambra1^+/+ (WT) and Ambra1^+/− sibling mice. Data were generated from three mice of each genotype for each time point. Values represent the mean ± SEM. Data were normalized with the housekeeping gene Gapdh. Statistical analysis was performed using Student’s t-test. *, P < 0.05. (D, E) Comparison of follicle proportion in ovaries of 3-month-old (D) and 10-month-old (E) Ambra1^+/+ and Ambra1^+/− mice. The proportion of follicles is displayed as the percentage of each follicle type over the total number of follicles (primordial, primary, secondary, antral, preovulatory, atretic and corpora lutea) over the total number of follicles. Values represent the mean ± SEM. Data were generated from three mice of each genotype for each time point, except for 3-month-old Ambra1^+/− mice (four ovaries). Statistical analysis was performed using Student’s t-test. *, P < 0.05