Fig. 2

KO of ambra1a and ambra1b reduces the reproductive capabilities of zebrafish in both sexes. (A) Representative histological analysis of 16-mpf ambra1b^−/− female, showing H&E staining of total body sagittal section. Scale bar, 5 mm. (B) Magnification of the dotted area of panel A, showing the ovary region. Scale bar, 400 μm. The lower panels show higher magnifications of the respective dotted areas (1–3), corresponding to different regions of the ovary. Scale bar, 200 μm. (C-J) Quantification of the reproductive performance of WT, ambra1a^−/− and ambra1b^−/− males (C-F) and females (G-J). Panels C and G show the percentage of spawning success in mutant male and female tests, respectively. Panels D and H show the percentage of fertilized eggs on the total number of eggs. Panels E and I show the mean number of eggs spawned in successful breeding events. Panels F and J show the percentage of survival of the offspring at 5 dpf. Males: ambra1a^−/− n = 4; ambra1b^−/− n = 4; WT n = 4. Females: ambra1a^−/− n = 4; ambra1b^−/− n = 3; WT n = 4. Error bars indicate SEM. Statistical analysis was performed using One way ANOVA. * P < 0.05; ** P < 0.01