Fig. 5

In situ confocal observation of the effects of 0.1% Triton X-100 treatment for 10 min on nuclear membrane and nuclear lamina. a, b Effects on nuclear membranes of HUVEC (a) and HepG-2 (b) cells stained with Dio (a fluorescent dye for lipids). c, d Effects on nuclear Lamina (a nuclear cytoskeleton beneath nuclear membrane) of HUVEC (c) and HepG-2 (d) cells stained with anti-Lamin (Lamin A + Lamin C) mAb and FITC-conjugated 2nd antibody. Upper panels: before treatment; bottom panels: 0.1% Triton X-100 treatment for 10 min. Left panels: DIC images; middle panels: fluorescent images of biomembranes (a, b) or lamina (c, d) in green; right panels: images merged from DIC and fluorescence images including DAPI in blue. The arrows in the upper panels of a and b indicate the extranuclear membranes stained by Dio which disappears after Triton treatment (the bottom panels of a and b). Scale bars: 20 μm (a–d)