Fig. 3

Morphological and size validation of the in situ isolation of nuclear proteins from adherent nuclei. a–c In situ confocal microscopic observation of cell nuclei treated with 0.01%, 0.1%, and 1% SDS, respectively without PBS washes. The nuclei were obtained from HUVECs pre-treated with 1% Triton X-100 for 10 min. Left panels: before treatments; right panels: 5 min, 1 min, and 30 s, respectively after treatments. Scale bars: 20 μm. d–f Size distributions of the entire cells, the intact nuclei after Triton X-100 treatment, and the nuclear debris/proteins after SDS treatment, respectively detected by DLS