Table 1 primer sequences for PCR
Gene | Forward primers (5′-3′) | Reverse primers (5′-3′) |
|---|---|---|
GAPDH | AACTTTGGCATTGTGGAAGG | ACACATTGGGGGTAGGAACA |
CD51 | AAGAGTTTGTTGCCGCCTTA | CAATGGCCACACACAGAGAC |
CD29 | AACTCCGACGCCTTTTCTTT | CCCCCATATTGCAAACAGAC |
CD105 | CTTCCAAGGACAGCCAAGAG | GTGGTTGCCATTCAAGTGTG |
Sca1 | CTGTGGTGGGGTGCTTTACT | GCCAGTGCAGAAGGGTAGAG |
Ki67 | AAGAGCAGGTTAGCACTGTTATGAA | TGCAGATGCATCAAACTTGG |
Caspase3 | GGGCCTGTTGAACTGAAAA | CCGTCCTTTGAATTTCTCCA |
Oct4 | CAGACCACCATCTGTCGCTTC | AGACTCCACCTCACACGGTTCTC |
SSEA1 | GCAGGGCCCAAGATTACTGAC | AAGCGCCTGGGCCTAAGAA |
KLF4 | AACATGCCCGGACTTACAAA | TTCAAGGGAATCCTGGTCTTC |
Sox2 | GTTCTAAGTGGTACGTTAGGCGCTTC | TCGCCCGGAGTCTAGCTCTAAATA |
cMyc | ACCCTCAAACTCCTGGTCCT | CAGGATGTAGGCGGTGGCTT |
Nanog | AACATGCCCGGACTTACAAA | ACCCTCAAACTCCTGGTCCT |
OPG | CTGCCTGGGAAGAAGATCAG | TTGTAAGCTGTGCAGGAAC |
Runx2 | CCCAGCCACCTTTACCTACA | TATGGAGTGCTGCTGGTCTG |
DMP1 | AGTGAGTCATCAGAAGAAAGTCAAGC | CTATACTGGCCTCTGTCGTAGCC |
BSP | GAGACGGCGATAGTTCC | AGTGCCGCTAACTCAA |
PPARG | GACAGGCATGGAAATGGAGT | CAGAAATCCTCCTGCCTCTG |
LPL | GGGCTCTGCCTGAGTTGTAG | CCATCCTCAGTCCCAGAAAA |
NF-M | GCCGAGCAGACCAAGGAGGCCATT | CTGGATGGTGTCCTGGTAGCTGCT |
NeuN | GTAGCGGTCCATACCAGGAA | CAGCCTGACTTCAGGGACTC |
GFAP | ACCTCGGCACCCTGAGGCAG | CCAGCGACTCAACCTTCCTC |
HIF-1α | TCAAGTCAGCAACGTGGAG | TATCGAGGCTGTGTCGACTG |
HIF-2α | CAGTACTCCCACAGGCCTGACTAAC | GACTGTCACACCGCTGCCATA |
VEGF | GCTCTCCACGATTTGACCAT | ATCCACCCACTAGGCAACAG |