Fig. 7

Effect of HIF-1/2α inhibition on AB-MSC spheroids formation. a Analysis of HIF-1α and HIF-2α expression in spheroids and monolayer by RT-qPCR (n = 3, **, p < 0.01; ***, p < 0.001, Student’s t-test, compared to the monolayer). b CCK-8 assay was used to assess cell viability under treatment with different concentrations of HIF-1/2α specific inhibitor (topotecan) and HIF-2α specific inhibitor (PT-2385) (n = 3, ***, p < 0.001, ANOVA test). c RT-qPCR was used to assess the expression of HIF-1/2α following HIFs inhibitor treatment (n = 3, ***, p < 0.001, ANOVA test). d After HIF-1/2α inhibitors treatment, the formation of spheroids was observed at 24 h. Scale bars = 100 μm. e The number and diameter of spheroids were evaluated after HIFs inhibition (n = 20, ***, p < 0.001, ANOVA test)