Fig. 6

Comparison of stemness between spontaneous and mechanical spheroids. a Morphological observation of spontaneous and mechanical spheroids (1000 cells) at 24 and 72 h. Scale bars = 100 μm. b The mRNA expression levels were analyzed between spontaneous and mechanical spheroids (n = 3). c RT-qPCR analysis of the expression of pluripotency markers in floating spheroids and adherent cells in low-adherence specific culture plates (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001, Student’s t-test, compared to mechanical spheroids or the adherent cells