Skip to main content
Fig. 5 | Biological Research

Fig. 5

From: Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors

Fig. 5

Neurogenic capability of AB-MSC spheroids. a Neurogenic differentiation of spheroids and monolayer for 14 d. Immunofluorescence showed positive staining for neurogenic markers NF-M (green), βIII tubulin (green), NeuN (red), and GFAP (green) in spheroid-derived cells. Scale bars = 50 μm. DAPI, 4',6-diamidino-2- phenylindole. (b) The RT-qPCR analysis of mRNA expression levels of NF-M, NeuN, and GFAP in spheroids and monolayer (n = 3). *, p < 0.05; **, p < 0.01, Student’s t-test, compared to the monolayer

Back to article page