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Fig. 4 | Biological Research

Fig. 4

From: Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors

Fig. 4

Osteogenic and adipogenic capability of AB-MSC spheroids. a After 14 d of osteogenic induction, ALP staining and ALP activity assay were used to evaluate ALP activity in spheroids and monolayer (n = 3). b The expression of osteogenic markers was analyzed via RT-qPCR on days 7 and 14 of osteogenic induction (n = 3). c Differences in osteogenic capacity between AB-MSC spheroids and bone marrow MSCs were compared by using RT-qPCR after 14 d of osteogenic differentiation (n = 3). d Oil-red O staining was used to assess lipid droplets after 10 d of adipogenic induction in spheroids and monolayers. The expression of adipogenic markers was analyzed via RT-qPCR (n = 3). Scale bars = 100 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.001, Student’s t-test, compared to the monolayer

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Biological Research

ISSN: 0717-6287

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