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Fig. 3 | Biological Research

Fig. 3

From: Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors

Fig. 3

Characterization of pluripotency in AB-MSC spheroids. a The expression of pluripotency markers Oct4 (red), Sox2 (green), KLF4 (red), and cMyc (red) in spheroids was evaluated via immunofluorescence. Scale bars = 100 μm. DAPI, 4',6-diamidino-2- phenylindole. b The expression of pluripotency markers and ESC markers (SSEA1 and Nanog) in spheroids and monolayer was analyzed via RT-qPCR (n = 3). *, p < 0.05; **, p < 0.01; ***, p < 0.001, Student’s t-test, compared to the monolayer

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Biological Research

ISSN: 0717-6287

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