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Fig. 2 | Biological Research

Fig. 2

From: Spontaneous spheroids from alveolar bone-derived mesenchymal stromal cells maintain pluripotency of stem cells by regulating hypoxia-inducible factors

Fig. 2

Analysis of cell proliferation and apoptosis in AB-MSC spheroids. a Immunofluorescence was used to assess the expression of cell proliferation marker Ki67 (red) and apoptosis marker Caspase3 (green) in spheroids. Scale bars = 100 μm. DAPI, 4',6-diamidino-2- phenylindole. b RT-qPCR analysis of Ki67 and Caspase3 expression in spheroids during 120 h culture (n = 3, ***, p < 0.001, ANOVA test). c Cell viability of spheroids and monolayer was measured by CCK-8 (n = 3, ***, p < 0.001, Student’s t-test, compared to the monolayer)

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Biological Research

ISSN: 0717-6287

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