Fig. 1
From: Disseminated intravascular coagulation phenotype is regulated by the TRPM7 channel during sepsis
TRPM7 modulates endotoxin-induced platelet and neutrophil adhesion to endothelial cells. Representative images from vehicle- and endotoxin-treated ECs exposed to saline solution (A), carvacrol (B), 2-APB (C), or non-transfected (E), transfected with a siRNA against TRPM7 (siRNATRPM7) (F) or transfected with a siRNA control (siRNANontarget) (G) and then cocultured with platelets for 24 h. Scale bar represents 100 μm. Platelets were stained by Vybrant Dio (green) and platelet adhesion was analyzed (D and H). (N = 5). Representative images from vehicle- and endotoxin-treated ECs exposed to saline solution (I), carvacrol (J), 2-APB (K), or non-transfected (M), transfected with a siRNA against TRPM7(siRNATRPM7) (N) or transfected with a siRNA control (siRNANontarget) (O) and then cocultured with neutrophils for 30 min. Scale bar represents 100 μm. Neutrophils were stained by Vybrant Did (red). Neutrophil adhesion was analyzed (L and P). (N = 5). Results were normalized against vehicle-exposed cells (control condition). Statistical differences were assessed by a two-way analysis of variance (ANOVA) followed by Tukey post hoc test. ***p < 0.001 and ****p < 0.0001, compared with the vehicle-treated condition in the saline- or non-transfected-condition. Results showed as mean ± SEM