Fig. 3
EPC-EXsmiR-17-5p infusion increases miR-17-5p levels, gastrocnemius muscle weight and structure integrity, while reduce apoptosis in hind limb of DHI mice. A Images showing PKH 26 labeled EPC-EXs (red) merged into muscle cell (Myod1, green). Scale bar, 20 μm. B The levels of miR-17-5p in hind limb muscle tissue after EPC-EXs and EPC-EXsmiR-17-5p administration. C Summary data of gastrocnemius muscle weight in DHI mice. D The structural integrity of ischemic muscles was measured by Masson’s staining. E Summary data of force generation capacity of muscle in DHI mice. F Representative images and summary data of cell apoptosis in hind limb of DHI mice. *p < 0.05 compared with the Sham group; #p < 0.05 compared with PBS group; +p < 0.05 compared with EPC-EXs group (B–F Two-way ANOVA, followed by Tukey’s post hoc test. Data represent the mean ± SEM, n = 8 mice per group)