Fig. 5

Expression of osteoblastogenesis and adipogenesis differentiation marker genes. Cells were treated with siRNA to deplete ZEB1 and ZEB2 mRNA expression, or Control non-targeting siRNA. A ZEB1 and B ZEB2 gene expression in control MSCs and MSCs treated with siRNA to knock-down ZEB1 or ZEB2 mRNA expression. All experiments were performed in at least in 4 donors. Data are expressed as mean ± SEM in arbitrary units. C RUNX2 and D ALPL gene expression in 7 day differentiated MSCs to osteoblast. E FABP4 and F Adiponectin gene expression in 7 day differentiated MSCs to adipocytes. All the experiments were performed with at least 4 donors. G and H Staining of calcium and lipid deposits in MSCs differentiated to osteoblasts and adipocytes. G Alizarin red staining of calcium deposits in MSCs differentiated to osteoblasts for 21 days. Cells were treated or not with siRNA to deplete ZEB1 and ZEB2 gene expression. Lower panel: alizarin red staining quantification expressed in arbitrary units. H Oil red staining of lipid deposits in MSCs differentiated to adipocytes for 14 days. Cells were treated or not with siRNA to deplete ZEB1 and ZEB2 mRNA expression Lower panel: oil red staining quantification expressed in arbitrary units. All the experiments were performed in at least 4 donors. Data are expressed as mean ± SEM in arbitrary units. ** p < 0.01, *** p < 0.001