Fig. 4

ZEB1 and ZEB2 expression during MSC differentiation to osteoblasts. A ZEB1 gene expression represented in arbitrary units along the differentiation procedure. B Correlation of the gene expression of ALPL and ZEB1 along the differentiation. Spearman r = 0.6977. p = 0.0006. C ZEB2 gene expression represented in arbitrary units along the differentiation. D Ratio of induction of ZEB1 and ZEB2. All the experiments were performed in at least 5 different donors. Data are expressed as means ± SEM. * p < 0.05, ** p < 0.01. E Output from one simulation of the computational modelling. The panel represents the ratio of the mRNA expression of ZEB1/ZEB2 along 21 (virtual) days of differentiation. F Output from one simulation of the computational modelling. The panel shows the increased ALPL mRNA along 21 (virtual) days of differentiation