Sperm DNA integrity does play a crucial role for embryo development after ICSI, notably when good-quality oocytes from young donors are used

Table 3 Incidence of overall DNA damage (alkaline Comet) and of double-stranded breaks (neutral Comet) in donor sperm, and percentages of sperm with low, medium or high DNA fragmentation

	Donor-donor cohort		Donor-infertile cohort		P-value
	Average ± SD	95% CI	Average ± SD	95% CI	P-value
Global incidence of DNA breaks (Alkaline comet OTM)	32.57 ± 6.86	[29.80–35.34]	32.89 ± 7.62	[30.23–35.55]	0.915
Percentage of sperm with low incidence of DNA damage (% low alkaline Comet)	32.71 ± 23.70	[22.93–42.49]	32.73 ± 24.30	[24.11–41.35]	0.999
Percentage of sperm with medium incidence of DNA damage (% medium alkaline Comet)	47.05 + 16.22	[40.35–53.74]	46.12 ± 15.98	[40.45–51.79]	0.916
Percentage of sperm with high incidence of DNA damage (%high alkaline Comet)	20.24 ± 18.15	[12.75–27.74]	21.18 ± 18.45	[14.64–27.72]	0.928
Incidence of double-stranded DNA breaks (Neutral comet OTM)	3.29 ± 2.27	[2.41–4.17]	3.32 ± 1.89	[2.66–3.98]	0.471
Percentage of sperm with low incidence of double-stranded DNA breaks (% low neutral Comet)	89.37 ± 17.37	[82.64–96.11]	89.44 ± 15.11	[94.17–94.71]	0.533
Percentage of sperm with medium incidence of double-stranded DNA breaks (%medium neutral Comet)	10.15 ± 16.50	[3.75–16.55]	10.18 ± 14.52	[5.11–15.24]	0.533
Percentage of sperm with high incidence of double-stranded DNA breaks (%high neutral Comet)	0.48 ± 1.11	[0.051–0.91]	0.44 ± 0.79	[0.17–0.72]	0.850

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