Fig.2

Reduction of OL-SCs exosomes secretion largely prevented the stimulatory effect of OL-SCs exosomes on OL cells migration and invasion in vitro. Exosome secretion was reduced by knocking down the key gene RAB27a that regulates the secretion and release of exosomes in OL-SCs cells. A, B RT-qPCR and Western blots were performed confirm effective silencing of RAB27a expression in OL-SCs cells. The right side of Figure B is the grayscale analysis statistical chart of WB (the ordinate is the target protein/GAPDH) C The concentration and size distribution of OL-SCs exosomes after RAB27a knocking down were confirmed by NTA. D The exosomes secreted by OL-SCs cells silenced by RAB27a gene inhibited the migration and invasion of OL cells by Transwell assay. E, F Quantitative analysis of migrating and invasive cells. (Three replicate wells were set in each group, which were repeated 3 times.10 random fields from three replicate Transwell were counted. n = 3) Scale bar, 64 μm. Data were expressed as the mean \(\pm\) SD, *p < 0.05, **p < 0.01, ***p < 0.001