Fig. 3

mtDNA injection (0.02 µg/µl, 2 µl) caused retinal cell damage, assayed with TUNEL staining after 7 days. A Representative retinal sections from the mtDNA retinal transfection group and normal control group. GCC, ganglion cell complex; INL, inner retinal nuclear layer; ONL, outer retinal nuclear layer. B Statistical analysis of the percentage of TUNEL-positive cells in each group. In each section, two areas 500 μm away from the optic nerve head, were selected for analysis. The number of TUNEL-positive cells was counted in each visual field and averaged. Only one section was chosen from each eye. n = 3 biological replicates in each group; **P < 0.01, scale bar: 50 μm