Fig. 7

Betahistine reduces vestibular dysfunction through inducing the expression of CTRP family members and activating the ERK1/2-AKT/PPARγ pathway in mice. Fifty-six mice were randomly divided into four groups and treated with normal saline, gentamicin, gentamicin + Betahistine, gentamicin + Betahistine + SCH772984, respectively. Then, CTRP1 (A) and CTRP12 mRNA (B) and protein levels (C and D), p-ERK1/2 protein levels (E and F), p-AKT protein levels (G) and PPARγ mRNA (H) and protein levels (I) were detected with RT-qPCR and western blotting, respectively. We also evaluated the accuracy of air righting reflex (J), the time of contact righting reflex (K) and the scores of head tilt and swimming behavior in mice (L). N = 7, Statistical differences were performed by using a one-way ANOVA. *P < 0.05, **P < 0.01