Table 1 Target gene, primer sequences and amplicon length used in the qPCR and Knockout study
Gene | Sequences of nucleotide (5′–3′) | Amplicon length (bp) | EMBL accession no. or reference |
|---|---|---|---|
EGR 1 | Forward: AGCTGTGCAGTGCAGTCCAACGAC Reverse: TAGTCGGGGATCATGGGAACCTG | 194 | XM_006070841.2 |
vWF | Forward: ATCGTAGGGGACTTCCAAGGTGG Reverse: CGGTCTCCAGGTATAGCCCTCTGG | 154 | 52 |
AKT | Forward: AAACCGTTACCTTGCTATG Reverse: TGCCCAGTTCGTTTCAGT | 159 | NM_174056. 3 |
RPS15A | Forward: AGGGCTGGGAAAATTGTTGTGAA Reverse: TGAGGGGATGGGAGCAGGTTAT | 104 | 24 |
CYP11A1 | Forward: AGACTTGGAGGGACCATGTAGC Reverse: TGCCTGGGTAATTCCTAAATTC | 117 | XM_025271874.1 |
TGF β1 | Forward: AACAATTCCTGGCGCTACCTC Reverse: TGCCGCACAACTCCAGTG | 92 | XM_006052063.2 |
3βHSD | Forward: AATCCGGGTGCTAGACAAAGT Reverse: CACTGCTCATCCAGAATGTCTC | 111 | XM_006049357.2 |
StAR | Forward: CTGCGTGGATTAACCAGGTTCG Reverse: CCAGCTCTTGGTCGCTGTAGAG | 84 | XM_006054485.2 |
EGR1 SgRNA | Forward:TAATACGACTCACTATAGGTCCATGGTGGGCGAATG Reverse: TTCTAGCTCTAAAACCATTCGCCCACCATGGAC | – | 26 |
EGR1 genomic detection | EGR1 Forward: CTACCCCAGCCTCGGTAGCA EGR1 Reverse: TCAGGTGCTCGTAGGGCTGC | Â | Â |