Fig. 5From: Deciphering the functional role of EGR1 in Prostaglandin F2 alpha induced luteal regression applying CRISPR in corpus luteum of buffaloGel image of genomic cleavage detection assay of luteal cells transfected with Cas9 and EGR1 SgRNA using Lipofectamine 2000. Lane 1, 50Â bp DNA ladder; Lane 2, negative control sample for EGR1 gene; Lane 3 sample without T7/E1 enzyme, Lane 4: sample, showing parent and both the cleaved bands after addition of T7/E1 enzymeBack to article page