Fig. 5

AMPK is required for testosterone-mediated glucose metabolism and hypertrophy. a Cardiomyocytes were transfected with siRNA-control or siRNA-AMPKα2 (20 nM) and protein levels were determined by Western blot. b Transfected cardiomyocytes were stimulated with 100 nM testosterone for 24 h and were then incubated with 2-NBDG for 20 min prior to glucose uptake measurements. c Cells were transfected and stimulated with testosterone 100 nM for 24 h and were evaluated the levels of HK2 protein by Western Blot. d Transfected cardiomyocytes were stimulated with 100 nM testosterone by 24 h and cellular area was measured using the fluorescent dye Rhodamine/Phalloidin and > 100 cells were analyzed in each condition. Data are expressed as mean ± SEM of at least three independent experiments. P-values were determined using t-test for Fig. 4a and ANOVA followed by Tukey’s post hoc test for the rest; *P < 0.05, **P < 0.01, ***P < 0.001 vs. control; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. testosterone