Fig. 3
From: Short-term perinatal oxygen exposure may impair lung development in adult mice
Lung histopathology. Lung sections were assessed by H&E staining in all the groups at four weeks (a 21%O2, b 40%O2 & c 100%O2) and 12 weeks of age (d 21%O2, e 40%O2 & f 100%O2) following neonatal oxygen exposure. Lungs did not demonstrate gross evidence of alveolar simplification (Scale bar: 100 µm). Radial alveolar count (RAC) (g) and mean linear intercept (MLI) (h) was estimated in all the groups (21%O2—grey bars; 40%O2—white bars; 100%O2—black bars; 400 × resolution). RAC was not significantly different in the groups at four weeks and 12 weeks in mice (g). However, MLI demonstrated a significant interaction concerning oxygen groups (p < 0.001; Two-way ANOVA). On multiple comparisons, exposure to 100%O2 soon after birth resulted in a significant increase in MLI at 4 and 12 weeks of age in mice (*p < 0.001 vs. 21%O2 & 40%O2 groups, Two-way ANOVA). (n = 6/group, each time-point)