Fig.2

Gastric cancer cells with REC8 depletion promoted chemotactic migration and tube formation of endothelial cells. a Real-time and b western blotting were performed to examine the REC8 expression at mRNA and protein level. c, d Conditional medium (CM) from BGC823 or AGS-1 cells infected with shCTL and shREC8 were used to be chemoattractant for HUVECs migration by transwell assays. ***p < 0.001 versus shCTL group by t-tests. Sar bar = 50um; e, f HUVECs were treated with conditional medium from BGC823 cells treated as indicated. Tube formation of HUVEC cells were visualized by phase contrast inverted microscope (100 ×), **p < 0.01 versus shCTL group by t-tests