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Fig. 2 | Biological Research

Fig. 2

From: Silencing of circular RNA ANRIL attenuates oxygen–glucose deprivation and reoxygenation-induced injury in human brain microvascular endothelial cells by sponging miR-622

Fig. 2

circ_ANRIL aggravates OGD/R-induced cell damage. a Upregulation of circ_ANRIL was induced by transfection with circ_ANRIL overexpressing plasmid. The expression of circ_ANRIL in OGD/R-treated HBMECs after plasmid transfection was detected by qRT-PCR. b The results of CCK-8 assay showed that overexpression of circ_ANRIL significantly aggravated the reduction of proliferation viability induced by OGD/R treatment in HBMECs. c The results of LDH assay showed that circ_ANRIL overexpression aggravated OGD/R-induced cell cytotoxicity. d The apoptosis rate of OGD/R-induced HBMECs was analyzed by flow cytometry. e The expression of Bcl-2 and Bax was analyzed by western blotting. The promotion effect of circ_ANRIL on OGD/R-induced apoptosis was verified by the changes in protein expression of Bcl-2 and Bax. **p < 0.01, ***p < 0.001. n = 3

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Biological Research

ISSN: 0717-6287

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