Table 1 The details of primers sequence and the annealing temperature
| Primer | Sequence (forward, reverse) | Annealing temperature |
|---|---|---|
| HPRT1 |
F: AATTACTTTTATGTCCCCTGTTGACTGG R: GCTATAAATTCTTTGCTGACCTGCTG | 63 |
| VEGF |
F: TTGCCTTGCTGCTCTACCTCCA R: GATGGCAGTAGCTGCGCTGATA | 62.5 |
| HIF-1a |
F: TGAGTTCGCATCTTGATAAGGC R: ACAAAACCATCCAAGGCTTTCA | 63 |
| N-cadherin |
F: GGACAGTTCCTGAGGGATCA R: GGATTGCCTTCCATGTCTGT | 62.5 |
| Vimentin |
F: GGTGGACCAGCTAACCAACGA R: TCAAGGTCAAGACGTGCCAGA | 62.5 |
| β-catenin |
F: AAAATGGCAGTGCGTTTAG R: TTTGAAGGCAGTCTGTCGTA | 62 |