Fig. 2

Myogenic differentiation of EGFP hiPSCs in vitro. a Experimental scheme for myogenic progenitor induction from EGFP hiPSCs followed by myogenic maturation. b Immunofluorescence analysis indicated expression of the early myogenic markers PAX7 and MYF5 at differentiation day 14. c Late myogenic markers, Desmin, MHC, and dystrophin were detected by on day 36 via immunofluorescence analysis. d Western blot analysis of myogenic markers at different time points during myogenic differentiation of EGFP hiPSCs (All data are presented as the mean ± SEM, n = 3 independent differentiation experiments). Scale bars = 100 μm