Fig. 3

Interference of MAPK1 suppresses cell viability, migration and invasion but induces apoptosis in gastric cancer cells. a, b The abundances of MAPK1 mRNA and protein in MKN-45 and MGC-803 cells were measured after transfection of sh-MAPK1-1, sh-MAPK1-2 or sh-NC via qRT-PCR and western blot. Cell viability (c, d), apoptosis (e), migration (f), invasion (g) and related protein levels (h, i) were determined in MKN-45 and MGC-803 cells transfected with sh-MAPK1-1, sh-MAPK1-2 or sh-NC via MTT, flow cytometry, transwell assay and western blot. sh-MAPK1: MAPK1 knockdown using shRNA; sh-NC: shRNA negative control. *P < 0.05 compared with sh-NC group