Fig. 2

Knockdown of LINC00483 inhibits cell viability, migration and invasion but promotes apoptosis in gastric cancer cells. a, b qRT-PCR assay was performed to analyze the transfection efficacy in MKN-45 and MGC-803 cells after transfection of sh-LINC00483-1, sh-LINC00483-2 or sh-NC. c, d Cell viability was measured in MKN-45 and MGC-803 cells transfected with sh-LINC00483-1, sh-LINC00483-2 or sh-NC by MTT. e Cell apoptosis was detected in MKN-45 and MGC-803 cells transfected with sh-LINC00483-1, sh-LINC00483-2 or sh-NC by flow cytometry. f, g Cell migration and invasion were determined in MKN-45 and MGC-803 cells transfected with sh-LINC00483-1, sh-LINC00483-2 or sh-NC by transwell assay. h, i The protein levels of c-Myc, Bax and MMP9 were measured in MKN-45 and MGC-803 cells transfected with sh-LINC00483-1, sh-LINC00483-2 or sh-NC by western blot. sh-LINC00483: LINC00483 knockdown using shRNA; sh-NC: shRNA negative control. *P < 0.05 compared with sh-NC group