Fig. 4

POU3F2 is a downstream target of miR-107 in melanoma cells. a Schematic diagram of a conserved putative miR-107 targeting site in 3′UTR of POU3F2 (WT). The site mutations were introduced to miR-107 targeting site of 3′UTR (MUT). b Luciferase reporter assay study of the interaction between miR-107 and 3′UTR of POU3F2. SH-4 cells were transfected with luciferase reporter genes conjugated with either wildtype (WT) or mutant (MUT) 3′UTR of POU3F2. 24 h later, the cells were transfected further with either miR-107 mimic or scramble control. Transfected cells were cultured for 48 h and then harvested for the measurement of luciferase activities in a plate reader. c Western blotting analysis of POU3F2 in cells transfected with either miR-107 or control miRNA. The representative image from at least three independent experiments was shown. d The densitometric analysis of POU3F2 expression was shown. The data were presented as mean ± SD of at least three independent experiments: (*p < 0.05, **p < 0.01, ***p < 0.001 as compared with control)