Fig. 6

SYVN1 was a target mRNA of miR-34a-5p. a The expression of SYVN1 in OA tissues and normal tissues was detected by qRT-PCR. b The expression of SYVN1 in OA cells stimulated 10 ng/ml IL-1β and OA cells. c The predicted binding sites of SYVN1 to miR-34a-5p sequence using starBase 2.0. d The luciferase activity of 293T cells cotransfected with miR-34a-5p and luciferase reporters containing SYVN1-wt or SYVN1-mut was detected using luciferase reporter assay. e The expression of SYVN1 was detected in the samples bound to the Ago2 antibody or IgG by RIP assay. f, g The mRNA f and protein g expression of SYVN1 was detected in OA cells (IL-1β) transfected with NC, miR-34a-5p, anti-NC and anti-miR-34a-5p by qRT-PCR and western blot. *P < 0.05