Fig. 5

Overexpression of miR-34a-5p reversed the effect of SNHG7 on OA cells. a The expression of miR-34a-5p was detected in OA cells transfected with vector, lncRNA SNHG7, lncRNA SNHG7 + NC and lncRNA SNHG7 + miR-34a-5p by qRT-PCR. b Cell proliferation was measured in OA cells (IL-1β) transfected with vector, lncRNA SNHG7, lncRNA SNHG7 + NC and lncRNA SNHG7 + miR-34a-5p after transfection 24 h, 48 h, 72 h by MTT assay. c Cell apoptosis was detected in OA cells (IL-1β) transfected with vector, lncRNA SNHG7, lncRNA SNHG7 + NC and lncRNA SNHG7 + miR-34a-5p by flow cytometry. d The protein expression of PCNA and cleaved-caspase 3 were measured in OA cells (IL-1β) transfected with vector, lncRNA SNHG7, lncRNA SNHG7 + NC and lncRNA SNHG7 + miR-34a-5p by western blot. e The protein expression of beclin1 and LC3 were measured in OA cells (IL-1β) transfected with vector, lncRNA SNHG7, lncRNA SNHG7 + NC and lncRNA SNHG7 + miR-34a-5p by western blot. *P < 0.05