Fig. 5

MiR-485-5p regulated PTC cell progression through modulating Raf1. a–i TPC1 and BCPAP cells were transfected with miR-NC, miR-485-5p mimics, miR-485-5p mimics + Vector, and miR-485-5p mimics + Raf1, respectively. a The expression of Raf1 was detected by qRT-PCR. b, c The protein level of Raf1 was detected by western blot in TPC1 and BCPAP cells, respectively. d, e Cell proliferation was detected by CCK-8 assay at 0 h, 24 h, 48 h and 72 h after transfection. f The migrated cells were measured by transwell assay. g The invaded capacity was evaluated by transwell assay after transfection. h, i EMT-related proteins (MMP9, E-cadherin, N-Cadherin and Vimentin) expression was detected by western blot. **P < 0.01