Fig. 2

SNHG1 functions as the endogenous sponge of miR-153-3p and inhibits its expression in SH-SY5Y cells. a Wild-type or mutant SNHG1 transcript containing putative complementary recognition site of miR-153-3p. b Luciferase activity of SH-SY5Y cells co-transfected with SNHG1-wt or SNHG1-mut reporter and miR-153-3p or miR-NC was determined by luciferase reporter assay. c Correlation between SNHG1 and miR-153-3p in SH-SY5Y cell lysates was determined by RIP analysis with Ago2 antibody, IgG antibody was used as a negative control. d RNA pull-down analysis was conducted in SH-SY5Y cells using biotin-labeled SNHG1, followed by the determination of miR-153-3p expression by qRT-PCR. e Expression level of miR-153-3p in SH-SY5Y cells transfected with pcDNA-SNHG1, si-SNHG1 or the corresponding controls was detected by qRT-PCR analysis. Expression levels of miR-153-3p in midbrain of MPTP-induced PD mice f and MPP⁺-treated SH-SY5Y cells g were measured by qRT-PCR analysis. **P < 0.01