Fig. 2

ICAII stimulates the differentiation of ADSCs to SCs through inhibiting let-7i. a qRT-PCR was performed to measure the level of let-7i in ADSCs treated with ICAII (10⁻⁷ mol/L). b ADSCs were transfected with let-7i mimic or negative control mimic and the level of let-7i was determined by qRT-PCR. c qRT-PCR was conducted to measure the mRNA levels of NGF, NT-3, S100β, and P75. d, e Western bolt was performed to measure the protein levels of NGF, NT-3, S100β, and P75. f–h let-7i mimic and negative control mimic were transfected into ADSCs treated with ICAII (10⁻⁷ mol/L). Western bolt and qRT-PCR were conducted to measure the expression levels of NGF, NT-3, S100β, and P75. *P < 0.05, ^#P < 0.05