Fig. 2
From: Neuroprotective effects of an Nrf2 agonist on high glucose-induced damage in HT22 cells
ROS production in cells cultured in different types of medium. HT22 cells were cultured in normal glucose medium (25 mM d-glucose, A), hypertonic medium (25 mM glucose + 25 mM mannitol, B) or high-glucose medium (50 mM d-glucose, C) for 0, 6, 12, 24, 48 or 72 h. ROS production was measured using the DCFH-DA fluorescent probe under a fluorescence microscope at a magnification of ×400 and semi-quantified using ImageJ. The fluorescence intensity was correlated with the ROS level. The fluorescence intensity was significantly increased over time in HT22 cells in the high-glucose medium group (C) compared with the hypertonic medium group (B) and normal glucose medium group (A). ROS reactive oxygen species, DCFH-DA dichlorodihydrofluorescein-diacetate