Fig. 4

Characteristic monochrome and merged images for CK8 (red) in ventral prostate (VP), dorsolateral prostate (DLP), and anterior prostate (AP) lobes of mice at 1, 3, and 6 months after immunization or infection. Nuclei are counterstained with DAPI (blue). a, b, d Naïve, CFA, and PBS groups. A single layer of luminal cells is present, and no proliferating cells are observed. c, e EAP and E. coli groups. Multiple layers of luminal cells positive for CK8+ (in red) are observed at the third and sixth month of modeling