Fig. 3

On E15. A large number of TBX4-immunopositive cells were distributed in the epithelium of the rectum and URS, and the anal membrane in normal group (a, b). A fistula was seen between the rectum and urethra in ARM group. The expression of TBX4 in anal membrane and the epithelium of the rectum were very weak (c, d). Red rectangles in a and c are shown at higher magnification in b and d. ×100 (a, c). ×400 (b, d). Black arrowheads: positive cells. URS urorectal septum, U urethra, R rectum